University of Missouri
Animal Reproductive Biology Group
Prather Laboratory

Meetings

IETS Annual Meeting 2017

Spate, A.M., K.M. Whitworth, C.W. O’Gorman, A.K. Byrne, R.S. Prather, K.D. Wells. 2017. The use of paired CRISPR guide RNAs and the Cas9 system does not always produced site specific deletions of gene sequence in porcine cell and embryo culture. International Embryo Transfer Society annual meeting Abs #26. Reprod. Fertil. Devel. 29:120.

Spate, L.D., J.A. Benne, S.L. Murphy, R.S. Prather. 2017. In vitro matured gilt oocytes can have equal or better developmental competence than sow oocytes with new maturation media. International Embryo Transfer Society annual meeting Abs #86. Reprod. Fertil. Devel. 29:150.

Mordhorst, B.R., S.N. Bogue, K.D. Wells, J.A. Green, R.S. Prather. 2017. Oxygen deprivation does not further augment mitochondrial membrane potential in pharmacologically treated fibroblasts for use in somatic cell nuclear transfer. International Embryo Transfer Society annual meeting Abs #30. Reprod. Fertil. Devel. 29:122.

SSR 2016

Spate, L.D., Y. Yuan, R.S. Prather, R.M. Roberts. 2016. A combination of three growth factors can enhance porcine embryo development during in vitro culture. Society for the study of Reproduction Annual Meeting, San Diego, CA. #343.

Redel, B.K., Y. Yuan, L.D. Spate, R.S. Prather, R.M. Roberts. 2016. FGF2, LIF and IGF-1 added together during maturation and culture improve somatic cell nuclear transfer embryo development and overall efficiency. Society for the study of Reproduction Annual Meeting, San Diego, CA. #345.

Large Animal Genetic Engineering 2016

Mordhorst, B.R., S.L. Murphy, J.A. Benne, B.K. Redel, L.D. Spate, K.D. Wells, J.A. Green, R.S. Prather. 2016. Reduced mitochondrial function and gene expression in porcine donor-fibroblasts treated with CPI-613 or PS48 does not negatively impact subsequent in vitro clone development after somatic cell nuclear transfer. Large Animal Genetic Engineering conference. Bethesda, MD September 19-20.

IETS Annual Meeting 2016

Mordhorst, B.R., S.L. Murphy, L.D. Spat e, R.M. Ross, K.D. Wells, J.A. Green, R.S. Prather. 2016. Reduction of mitochondrial function, proliferation, and gene expression in fibroblasts donor-cells for use in SCNT by CPI-613 and PS48. International Embryo Transfer Society annual meeting. Reproduction, Fertility and Development 28:141-142.

Spate, L.D., B.K. Redel, R.S. Prather. 2016. Early porcine embryo energy preference and subsequent development. International Embryo Transfer Society annual meeting. Reproduction, Fertility and Development 28:170.

Benne, J.A., L.D. Spate, B.M. Elliott, S. Murphy, R.S. Prather. 2016. In low oxygen culture, is hypotaurine necessary for in vitro development of porcine embryos? International Embryo Transfer Society annual meeting. Reproduction, Fertility and Development 28:171.

Whitworth, K.M., S.L. Murphy, J.A. Benne, L.D. Spate, E. Walters, W.J. Raymond, S.L. Nyberg, K.D. Wells, R.S. Prather. 2016. Genome editing of somatic cell nuclear transfer derived zygotes by CRISPR/Cas9 guide RNA injection. International Embryo Transfer Society annual meeting. Reproduction, Fertility and Development 28:142-143.

Redel, B.K., L.D. Spate, B. Elliott, M. Paczkowski, R.L. Krisher, R.S. Prather. 2016. Porcine embryos utilize small amounts of pyruvate, lactate and glucose in vitro. International Embryo Transfer Society annual meeting. Reproduction, Fertility and Development 28:171.

American Society of Reproductive Immunology

Whitworth, K.M., J.A. Benne, B.P. Beaton, L.D. Spate, S.L. Murphy, M.S. Samuel, E.M. Walters, C.N. Murphy, K.D. Wells, R.S. Prather. 2015. Using the CRISPR/Cas9 system to edit specific genes in pig zygotes. American Society of Reproductive Immunology, Kingston, ON, Canada. June 4.

Whyte: CRISPR/Cas9-Directed inactivation of porcine interleukin-1β as a model to study endometrial receptivity and conceptus attachment

SSR 2015

Whitworth, K.M., J.A. Benne, L.D. Spate, S.L. Murphy, J.A. Richt, E. Walters, K.D. Wells, R. S. Prather. 2015. Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or changing the male:female sex ratio in pigs. Society for the Study of Reproduction annual meeting. #444.

Graduate Forum 2015

Mordhorst: Reduction of mitochondrial function in fibroblast donor-cells for use in SCNT by CPI-613 and PS48

IETS Annual Meeting 2014

Swine in Biomedical Research Conference 2014

Beaton: CRISPR/Cas9 mediated gene staking in swine

Zhao: Bioengineering of the mammary gland of pig: increased pro-peptide processing of Factor IX in the milk of transgenic pigs by co-expression of soluble FURIN

Whitworth: Use of CRISPR/Cas9 system to produce pigs with a genetically modified CD163 gene by somatic cell nuclear transfer with in vitro derived oocyte

Whyte: Epigenetic regulation of gene expression by DNA methylation in porcine nuclear transfer donor cells and embryos produced by SCNT, in vivo and in vitro fertilization.

Spate: Altering donor cell metabolism increases development of cloned embryos to the blastocyst stage

Lichtenauer: Fibroblast growth factor-8 CRISPR/Cas9 mediated gene targeting in swine

Graduate Forum 2014

Redel: Preimplantation embryo development in vitro is improved with glycine supplementation

A NOVEL METHOD TO INCREASE THE DEVELOPMENTAL POTENTIAL OF ACTIVATED OOCYTES BY USING Zn2+ CHELATOR, TPEN (N, N, N’, N’-tetrakis (2pyridylmethyl) ethylene diamine)

Oxamflatin Treatment Enhances Nuclear Reprogramming by Inhibiting XIST Expression and Reducing DNA Methylation in Porcine SCNT Embryos

PAG 2013

TET family is responsible for the appearance of 5-hydroxymethylcytosine during porcine embryo development

SSR 2013

TRIM28, SETDB1, and p53 Expression in Porcine Preimplantation Embryos

Preimplantation Embryo Development is Dependent on Glycine Metabolism

Oxamflatin Treatment Enhanced Porcine SCNT Embryo Development and Decreased DNA Methylation

Effect of WTAP Gene Expression during in vitro Maturation on the Development of Porcine Embryos in vitro

IXA 2013

Xenotransplantation resources available through the National Swine Resource and Research Center

Tahoe City- Transgenic Animal Conference 2013

Minimum length of homology in a donor DNA to facilitate homologous recombination during ZFN mediated gene targeting

Transcriptome analysis of pig nuclear transfer blastocysts treated with histone deacetylase inhibitors post-fusion and activation

SSR 2012

The effect of cysteine proteinase inhibitors on in vitro maturation of porcine cumulus-oocyte-complexes

IETS Annual Meeting 2012

DYNAMICS OF TET FAMILY DURING PREIMPLANTATION DEVELOPMENT OF PORCINE EMBRYOS

The histone deacetylase inhibitor 4-iodo-Suberoylanilide Hydroxamic Acid (I-SAHA) improves total cell number in pig nuclear transfer blastocysts

Regulation of Mitochondrial DNA Replication during Oocyte Maturation by Follicular Fluid, EGF, and Neuregulin 1 affects Oocyte Maturation and Development

The in vivo developmental potential of porcine skin-derived progenitors (SKPs)

GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR INCREASES DEVELOPMENTAL POTENTIAL OF PORCINE EMBRYOS IN VITRO

Altered mRNA Transcript Expression of In Vitro vs. In Vivo Matured Porcine Oocytes

Inhibition of Cysteine Proteinases During in vitro Oocyte Maturation Improves Development of Porcine Cumulus-Oocyte-Complexes

Treatment of Donor Cells with Xenopus Oocyte Extract Enhanced SCNT Embryo Development

SUPPLEMENTATION WITH FOLATE IN VITRO INCREASES TROPHECTODERM AND TOTAL CELL NUMBER IN IN VITRO DERIVED PORCINE BLASTOCYSTS

PWSA 2012

Genomic and cytogenetic characterization of the porcine imprinted and three non-imprinted domains orthologous to the human Prader-Willi syndrome chromosome region

IETS Annual Meeting 2011

Posters

High Throughput Vitrification of In VivoFertilized andIn Vitrocultured Porcine Embryos

N-METHYL-D-ASPARTIC ACID and HOMOCYSTEINE CAN BE USED TOGETHER AS A REPLACEMENT FOR BSA IN EARLY PORCINE EMBRYO CULTURE

Arginine Supplementation In Vitro Increases Porcine Embryo Development and Affects mRNA Transcript Expression

DNA methylome analysis in nuclear transfer donor cells and its relationship to cloning efficiency in swine

IETS Annual Meeting 2010

Scriptaid and MG132 Improve Development of Porcine Embryos Reconstructed by Somatic Cell Nuclear Transfer

IETS Annual Meeting 2009

BIOENGINEERING OF THE MAMMARY GLAND OF LIVESTOCK: INCREASED PROPEPTIDE PROCESSING OF FACTOR IX IN THE MILK OF TRANSGENIC PIGS BY CO-EXPRESSION OF FURIN